Endocytosis and trafficking
Endocytosis is a fundamental process in which eukaryotic cells internalise molecules and macromolecules via deformation of the membrane and generation of membrane-bound carriers. Its primary role is to regulate the uptake of nutrients, however, endocytosis also plays a primary role in evolutionary conserved processes such as the regulation of plasma membrane protein activity (i.e. signal-transducing receptors, small molecule transporters and ion channels), cell motility and mitosis. The macromolecular nature of the material transported by endocytosis makes this route one of the most important targets for nanomedicine. Indeed, many nanoparticles have been customised to enter cells through endocytosis and deliver their cargo within the cell. We have studied this process for several years and applying new methodologies to understand its implications in drug delivery. We are studying how size, shape and surface topology affect the way cell membrane deform and consequently internalise the material. We correlate these with intracellular trafficking and cellular signalling. We apply both fast live imaging and mathematical modelling to understand these interactions. From one side, we engaged with theoreticians in past such as Ramin Golestanian at Oxford and now started collaborating with Daan Frenkel at Cambridge to use modelling to understand membrane/nanoparticle interaction. From the other side, we have been working with cell biologists to access new tools to study endocytosis including Stephen Renshaw and Elisabeth Smythe at Sheffield as well as Frances Brodsky and Emmanuel Boucrot at UCL.
- J. D Robertson, G. Yealland, M. Avila-Olias, L. Chierico, O. Bandman, S. A Renshaw, G. Battaglia pH-sensitive tubular polymersomes: formation and applications in cellular delivery ACS Nano, 2014, 8, 4650–4661
- A. Akinc & G. Battaglia Exploiting endocytosis for nanomedicines. Cold Spring Harbor Perspectives in Biology, 2013, 5 (11), a016980
- I. Canton and G. Battaglia Endocytosis at the nanoscale Chem. Soc. Rev., 2012, 41, 2718-2739
- A. Chaudhuri, G. Battaglia, R. Golestanian Effect of interactions on the cellular uptake of nanoparticles Phys. Biol. 2011, 8, 046002 (9pp)
- M. Massignani, C. LoPresti, A. Blanazs, J. Madsen, S. P. Armes, A. L. Lewis and G. Battaglia Controlling cellular uptake by surface chemistry, size and surface topology at the nanoscale Small, 2009, 5 (21), 2424 – 2432
- H. Lomas, M. Massignani, K. A. Abdullah, I. Canton, C. Lo Presti, S. MacNeil, J. Du, A. Blanazs, J. Madsen, S. P. Armes, A. L. Lewis, G. Battaglia Non-cytotoxic polymer vesicles for rapid and efficient intracellular delivery Faraday Discuss. 2008, 139, 143 - 159.
- H. Lomas, I. Canton, S. MacNeil, J. Du, S.P. Armes, A.J. Ryan, A.L. Lewis and G. Battaglia Biomimetic pH Sensitive Polymersomes for Efficient DNA Encapsulation and Delivery, Adv. Mater. 2007, 19, 4238–4243