Microscopic techniques enable real-space imaging of complex biological events and processes. They have become an essential tool to confirm and complement hypotheses made by biomedical scientists and also allow the re-examination of existing models, hence influencing future investigations. Particularly imaging live cells is crucial for an improved understanding of dynamic biological processes, however, live cell imaging is often limited by the necessity to introduce probes within a cell without altering its physiological and structural integrity. In collaboration with Jim Thomas at Sheffield and more recently with Yupeng Tian and Xiaohe Tian at Anhui University, we have tested and validated new luminescent probes to gather structural information of DNA in live cells. Similarly exploiting our understanding of nanoparticle endocytosis we have also developed a new approach to delivery almost any probes within live cells exploiting natural cellular endocytosis. We can now functionally image live cells by highlighting their cellular membrane, nuclei or more specific sub-cellular structures by delivering antibodies. See our gallery for examples
Live fibroblast with actin (Red) and mitochondria (cyan) labelled using polymersomes (Chierico et al. Sci Reports 2014, 4, 6056)
Live fibroblast with actin (Red) labelled using polymersomes (Chierico et al. Sci Reports 2014, 4, 6056)
Live fibroblasts in 3D imaged after delivery of membrane probes (red) and RNA stain (green) (Massignani et al PLoS One 2010, 5 (5) e10459)
Live fibroblast with actin (Red) and PiP2 lipids (green) labelled using polymersomes (Chierico et al. Sci Reports 2014, 4, 6056)
- L. Chierico, A. S. Joseph, A. L. Lewis and G. Battaglia Live cell imaging of cytoskeleton interactions and membrane topology Sci. Rep. 2014, 4, 6056
- M. R. Gill, D. Cecchin, M. G. Walker, R. S. Mulla, G. Battaglia, C. Smythe, J. Thomas Targeting the endoplasmic reticulum with a membrane-interactive luminescent ruthenium (II) polypyridyl complex Chem. Sci., 2013, 4, 4512-4519
- J. Madsen, I. Canton, N. Warren, E. Themistou, A. Blanazs, B. Ustbas, X. Tian, R. Pearson, G. Battaglia, A. Lewis, S. P. Armes Nile blue-based nano-sized pH sensors for simultaneous far-red and near-infrared live bioimaging J. Am. Chem. Soc. 2013, 135, 14863–14870
- I. Canton and G. Battaglia Polymersomes-mediated delivery of fluorescent probes for targeted and long term imaging in live cell microscopy Meth. Mol. Biol., 2013, 991:343-51
- I. Canton, M. Massignani, N. Patikarnmonthon, L. Chierico, J. Robertson, S. A. Renshaw, N. J. Warren, J. P. Madsen, S. P. Armes, A. L. Lewis, G. Battaglia* Fully synthetic polymer vesicles for intracellular delivery of antibodies in live cells FASEB J.2013, 1 , 98-108.
- M. Gill, G. Battaglia, C. Smythe and J. Thomas Dual function ruthenium(II) DNA light-switches: cellular imaging and cytotoxicity ChemBioChem 2011, 12 (6), 877-880
- X. Tian, I. Canton, M. Gill, J. Thomas and G. Battaglia Live cell Luminescence Imaging as a Function of Delivery Mechanism ChemBioChem 2011, 12 (4), 548–55
- K. S. Kung, I. Canton, M. Massignani, G. Battaglia*, and A. M. Donald* The development of anisotropic behaviours of 3T3 fibroblasts on microgrooved patterns Eur. Phys. J. E 2011, 34(3), 1-9.
- M. Massignani, I. Canton, T. Sun, V. Hearnden, S. MacNeil, A. Blanazs, S. P. Armes, A. Lewis, and G. Battaglia Enhanced fluorescence imaging of live cells by effective cytosolic delivery of probes PLoS One 2010, 5 (5) e10459
- M. R. Gill, J. Garcia-Lara, S. J. Foster, C. Smythe, G. Battaglia and J. A. Thomas A Ruthenium(II) Polypyridyl Complex for Direct Imaging of DNA Structure in Living Cells Nature Chem. 2009, 1, 662 - 667