The Virus is one of most fascinating biological structures. It is not a living organism but its complexity is very close to whole cells and their structure exhibits high order and moreover is completely dedicated to one and one only function enter live cells to create more viruses. Such a static nature has allowed structural biologists and virologists to study their molecular arrangement with high detail and indeed we have now an atlas of morphologies where we can take inspiration for constructing similar complex systems, yet fully synthetic. In the last years, we have done so using the polymersome as canvas and the ability of copolymers to form domains on their surface. As showed in the figure on the right, we have been able to replicate specific virus feature using our supramolecular engineering approaches. We have combined this with effective encapsulation of nucleic acids and their delivery into cells (See video below). This has given us the ability to deliver almost any nucleic acids and in all effect replicating viral infection.
- L. Ruiz-Perez, L. Messager, J. Gaitzsch, A. Joseph, L. Sutto, F. L. Gervasio and G. Battaglia Molecular engineering of polymersome surface topology Science Adv. 2016, e1500948
- J.D Robertson, G. Yealland, M .Avila-Olias, L. Chierico, O. Bandman, S. A. Renshaw ,G. Battaglia pHsensitive tubular polymersomes: formation and applications in cellular delivery ACS Nano, 2014, 8, 4650–4661
- L. Wang, L. Chierico, D. Little, N. Patikarnmonthon, Z. Yang, M. Azzouz, J. Madsen, S. P. Armes, G. Battaglia* Encapsulation of Biomacromolecules within Polymersomes via Electroporation Angew. Chem. Int. Ed. 2012, 51, 11122-11125.
- G. Battaglia, C. LoPresti, S. Forster M. Massignani, J. Madsen, N. J. Warren, S. P. Armes, C. Vasilev, J. K. Hobbs, S. Chirasatitsin, A. Engler Wet nano-scale imaging and testing of polymersomes Small 2011, 7, (14), 2010–2015
- C.LoPresti, M.Massignani, C.Fernyhough, A.Blanazs, A.J.Ryan, J.Madsen, N.J.Warren, S.P.Armes, A.L. Lewis S. Chirasatitsin, A. Engler, G. Battaglia Controlling polymersomes surface topology at the nanoscale by membrane confined polymer/polymer phase separation ACS Nano 2011, 5 (3),1775–1784
- H. Lomas, J. Du, I. Canton, J. Madsen, N. Warren, S. P. Armes, A. L. Lewis and G. Battaglia Efficient Encapsulation of Plasmid DNA in pH-Sensitive PMPC-PDPA Polymersomes: Study of the Effect of PDPA Block Length on Copolymer-DNA Binding Affinity Macromol. Biosci., 2010, 10 (5), 513 - 530
- H. Lomas, I. Canton, S. MacNeil, J. Du, S.P. Armes, A.J. Ryan, A.L. Lewis and G. Battaglia Biomimetic pH Sensitive Polymersomes for Efficient DNA Encapsulation and Delivery, Adv. Mater. 2007, 19, 4238–4243