Topology of cell adhesion
We have been successful in designing materials whose surface topology (i.e. how different ligands are clustered together) is controlled. In collaboration with Adam Engler from UCSD, we demonstrated that adhesion of human mesenchymal progenitor (hES-MP) cells is dependent on foam surface topology and chemistry but is independent of porosity and interconnectivity (see figure 1). Most importantly, we demonstrated that heterogeneously spaced adhesive nano-domains are more efficient in guiding mesenchymal stem cell adhesion and differentiation. Adhesive patches that better reflect the adhesive spacing found in ECM allowed for the greatest amount of cell spreading irrespective of scaffold architecture. More recently and in collaboration with Marcelo Rivolta at Sheffield, we are applying this principle to neuronal progenitors with the aim to control the regeneration of the otic nerve.
- P. Viswanathan, E. Themistou, K. Ngamkham, G. Reilly, S. Armes, G. Battaglia Controlling Surface Topology and Functionality of Electrospun Fibers using Amphiphilic Block Copolymers to Direct Mesenchymal Progenitor Cell Adhesion Biomacromolecules 2015, 16(1):66-75
- P. Viswanathan, M. Ondeck, S. Chirasatitsin, K. Nghamkham, D. Cecchin, G. C. Reilly, A. J. Engler and G. Battaglia 3D Surface Topology Guides Stem Cell Adhesion and Differentiation Biomaterials 2015, 52, 140–147
- P. Viswanathan, S. Chirasatitsin, K. Ngamkham, A. Engler, and G. Battaglia Cell instructive microporous scaffolds through interface engineering J. Am. Chem. Soc. 2012, 134 (49), 20103–20109